5/18/2023 0 Comments Bangs lab quickcal![]() ![]() phycoerythrin-PE or fluorescein isothiocyanate-FITC), that make the reaction detectable. The use of antibodies in immunodiagnostic kits generally implies the conjugation of these proteins with other molecules, such as chromophores or fluorochromes (i.e. In therapy, there are numerous monoclonal antibodies licensed for the treatment of various diseases, like cancer, allergy and autoimmune diseases. ![]() In basic research, they are primarily used for staining both surface and intracellular proteins, like membrane receptors and cytokines. Monoclonal antibodies are glycoproteins containing uniform variable regions that confer a high specificity for a single epitope, favoring their use not only in scientific research, but also in immunodiagnostic and therapy. Our data demonstrated the feasibility of the flow cytometric method as a standard quality control of immunoassay kits. The MESF analysis, as well as geometric mean evaluation by traditional flow cytometry, showed a decrease in the values for all conjugates during the study and were indispensable tools to validate the results of stability tests. Coefficients of variation (CVs) showed that this parameter can be used to determine better coupling conditions, such as homogenous coupling. The differences were observed between manufactures and lots from both anti-IgG-PE and anti-HBSAg-PE conjugates. Our results showed that there was a great difference in the fluorescence intensities between the conjugates studied. Their fluorescence intensities were followed for a period of 18 months. We have coupled microspheres with anti-IgG-PE and anti-HBSAg-PE conjugates from distinct manufactures and/or different lots and evaluated by flow cytometry. In this article, we demonstrate a new flow cytometric protocol to evaluate conjugates by molecules of equivalent soluble fluorochromes (MESF) and by traditional flow cytometric analysis. The development of more sensitive quality control procedures than spectrophotometry is essential to assure the use of better fluorescent conjugates since the fluorescent conjugates are critical reagents for a variety of immunodiagnostic kits. The use of antibodies in immunodiagnostic kits generally implies the conjugation of these proteins with other molecules such as chromophores or fluorochromes. ![]()
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